De novo assembly and annotation of the whole transcriptome of Oratosquilla oratoria

摘要

As the representative species of Stomatopoda, Oratosquilla oratoria can tolerate the complex benthic environment. In this study, our goal was to develop the transcriptomic resource of O. oratoria that would support adaptation mechanism studies. We generated the whole transcriptome of O. oratoria from combined tissues (eyestalk, muscle, sexual and viscus) using Hiseq technology. A total of 51,305,284 high-quality clean reads were assembled to produce 59,054 non-redundant transcripts with a mean length of 987 nt using Trinity and CORSET software. Among the predictable unigenes, a total of 32,451 unigenes were annotated based on protein databases. Finally, we predicted the coding sequences of 31,822 unigenes and obtained 19,057 SSRs in the present study. The present study will provide an important resource and foundational understanding for future genomic research of O. oratoria.