A Scalable Adenovirus Production Process from Cell Culture to Purified Bulk ProductGE Healthcare describes how to improve virus load capacity and purity

摘要

Adenovirus (AdV) vectors are commonly used in cancer gene therapy trials, evaluated in gene therapy, and used in vaccines for various diseases. In this study, we have combined technical evaluation of process steps and process economy calculations, from AdV production in cell culture to purified bulk product in up to 10 L scale18 Human AdV5, expressing the green fluorescent protein (GFP), was used for process development. The cells were lysed using Tween 20 as an alternative to Triton X-100 that is now on the authorization list (Annex XIV) of Registration, Evaluation, Authorization, and Restriction of Chemicals (REACH).