Process for production of recombinant adenovirus purification process of recombinant adenovirus from a biological medium purified viral preparation pharmaceutical composition and the use of I.Odixanol - 5,5 '- (2 - hydroxypropyl - 1,3 - propanottl) - bis n, n' - bis (2,3 - didroxipropil - 2,4,6 - triode - 1,3 - benzenocarboxamida

摘要

Production of recombinant adenovirus (A) comprises: (a) introducing viral DNA into a culture of packaging cells, and (b) harvesting (A) produced after their release into the supernatant. Also claimed are: (1) purification of (A) from a medium by chromatography on a strong anion exchanger; (2) (A) prepared as described above; (3) use of iodixanol (I; 5,5'-[(2-hydroxypropane-1,3-diyl)bis(acetylamino)] bis[N,N'-bis(2,3-dihydroxypropyl) -2,4,6-triiodobenzene-1,3-dicarboxamide) for purification of adenovirus, and (4) purification of adenovirus from medium by: (i) ultracentrifugation; (ii) dilution or dialysis, and (iii) anion-exchange chromatography.