A Pdgf-c(CreERT2) knock-in mouse model for tracing PDGF-C cell lineages during development

摘要

PDGF-C, a member of the platelet-derived growth factor (PDGF) family, plays important roles in the development of craniofacial structures, the neural system, the vascular system, and tumors. PDGF-C could also be required for the regulation of certain types of stem or progenitor cells as suggested by its expression in the regions where these cells are located. To further characterize the role of PDGF-C in development, we generated a Pdgf-c(CreERT2) mouse strain, in which a tamoxifen-inducible Cre (CreERT2) cDNA was specifically targeted into the Pdgf-c genomic locus and controlled by the endogenous Pdgf-c regulatory elements. We also showed that Cre activity in this mouse strain could be specifically induced by tamoxifen, which allowed the fate of PDGF-C-expressing cells to be traced at various stages of development. Using this model system, we demonstrated for the first time that PDGF-C-expressing cells could be multipotent, generating multiple cell lineages required for the formation of the cerebellum. Therefore, the Pdgf-c(CreERT2) mouse strain generated in this study will be a valuable transgenic tool for exploring the function of PDGF-C in development and stem cell biology.