In Vitro Inhibition of alpha-Amylase and alpha-Glucosidase by Extracts from Psiadia punctulata and Meriandra bengalensis

摘要

Background. This research assessed the in vitro antidiabetic activity and phytochemical constituents of the traditionally used medicinal plants, Psiadia punctulata andMeriandra bengalensis. Method. The leaves of both plants were subjected to cold extraction method using 70% ethanol and hot Soxhlet extraction using n-hexane, chloroform, methanol, and distilled water. The extracts were studied for their effect on glucose transport across yeast cells and inhibition of alpha-amylase and alpha-glucosidase enzyme activities. Thinlayer chromatographic analysis of ethanol extract was also undertaken. Results. The results of yeast glucose uptake assay revealed that extracts from both plants had a maximum increase in glucose uptake at the 25mM glucose concentration with a maximum dose of 2000 mu g/ml plant extract. The ethanol extract of P. punctulata and aqueous extract of M. bengalensis showed a high activity of 68% and 96%, respectively, at 25mM and 2000 mu g/ml of glucose and extract concentration. P. punctulata exerted peak inhibition activity of alpha-amylase of 37.5 +/- 3% mg/dl (IC50 = 0.523 mg/dl) for methanol and distilled water extract at 0.5 mg/dl, respectively. M. bengalensis methanol extract exhibited the highest inhibition activity of 38 +/- 8 % mg/dl (IC50 = 0.543 mg/dl) at 0.5 mg/dl. In the alpha-glucosidase inhibition assay, the methanolic extract of P. punctulata exhibited the highest inhibitory activity of 17.29 +/- 9% mg/dl (IC50 = 0.761 mg/dl) at 0.5mg/dl. The chloroform extract of M. bengalensis had the highest inhibitory activity of 30 +/- 5% mg/dl (IC50 = 0.6mg/dl) at 0.5 mg/dL. Phytochemical analysis of the different extracts of P. punctulata and M. bengalensis revealed the presence of flavonoids, alkaloids, tannins, saponins, phytosterols, and carbohydrates. Thin-layer chromatography analysis of ethanolic extract of both plants indicated presence of 15 and 17 spots for P. punctulata and M. bengalensis respectively. Conclusion. P. punctulata and M. bengalensis extracts have moderate inhibitory activity against pancreatic alpha-amylase and relatively low inhibitory activities against alpha-glucosidase. The observed effects may be associated with the presence of flavonoids, saponins, and alkaloids. Additional in vivo analysis, toxicological studies, isolation, and structural characterization of the phytomolecules identified in this study and molecular docking studies should be undertaken.