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Quantitative chemical biosensing by bacterial chemotaxis in microfluidic chips

机译：细菌化学在微流体芯片中的定量化学生物溶解

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Summary > Whole‐cell bacterial bioreporters are proposed as alternatives to chemical analysis of, for example, pollutants in environmental compartments. Commonly based on reporter gene induction, bioreporters produce a detectable signal within 30 min to a few hours after exposure to the chemical target, which is impractical for applications aiming at a fast response. In an attempt to attain faster readout but maintain flexibility of chemical targeting, we explored the concept for quantitative chemical sensing by bacterial chemotaxis. Chemotaxis was quantified from enrichment of cells across a 600 μm‐wide chemical gradient stabilized by parallel flow in a microfluidic chip, further supported by transport and chemotaxis steady state and kinetic modelling. As proof‐of‐concept, we quantified Escherichia coli chemotaxis towards serine, aspartate and methylaspartate as a function of attractant concentration and exposure time. E. coli chemotaxis enrichment increased sharply between 0 and 10 μM serine, before saturating at 100 μM. The chemotaxis accumulation rate was maximal at 10 μM serine, leading to observable cell enrichment within 5 min. The potential application for biosensing of environmental toxicants was investigated by quantifying chemotaxis of Cupriavidus pinatubonensis JMP134 towards the herbicide 2,4‐dichlorophenoxyacetate. Our results show that bacterial chemotaxis can be quantified on a scale of minutes and may be used for developing faster bioreporter assays. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>摘要</ title> >全 - 将细胞细菌生物投递者作为化学分析的替代品，例如，环境隔间中的污染物。通常基于报道基因诱导，Biooreporters在暴露于化学靶标后30分钟内产生可检测的信号，这对于旨在快速反应的应用是不切实际的。试图达到更快的读数，但保持化学靶向的灵活性，我们探索了细菌趋化性的定量化学感测的概念。通过通过在微流体芯片中的平行流动稳定的600μm宽的化学梯度富集细胞来量化趋化性，通过传输和趋化性稳态和动力学建模进一步支持。作为概念证据，我们将大肠杆菌（Che）趋向于丝氨酸，天冬氨酸和甲基己二酸盐，作为引诱剂浓度和暴露时间。 e。 Coli </ i>趋化性富集在0和10μm丝氨酸之间急剧增加，在100μm下饱和。趋化性累积率最大化为10μm丝氨酸，导致可观察到的细胞富集在5分钟内。通过量化趋化性的趋化化趋向于除草剂2,4-二氯苯苯甲酸酯来研究对环境毒物生物沉积的潜在应用。我们的结果表明，细菌化学趋化性可以在分钟的等级上量化，并且可用于制定更快的生物功率测定。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology》</a> <b style="margin: 0 2px;">|</b><span>2018年第1期</span><b style="margin: 0 2px;">|</b><span>共18页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Roggo Clémence&option=202" target="_blank" rel="nofollow">Roggo Clémence;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Picioreanu Cristian&option=202" target="_blank" rel="nofollow">Picioreanu Cristian;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Richard Xavier&option=202" target="_blank" rel="nofollow">Richard Xavier;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mazza Christian&option=202" target="_blank" rel="nofollow">Mazza Christian;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=van Lintel Harald&option=202" target="_blank" rel="nofollow">van Lintel Harald;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=van der Meer Jan Roelof&option=202" target="_blank" rel="nofollow">van der Meer Jan Roelof;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Fundamental MicrobiologyUniversity of LausanneLausanne Switzerland 1015;</p> <p>Department of Biotechnology Faculty of Applied SciencesDelft University of TechnologyDelft 2629 HZ The Netherlands;</p> <p>Department of MathematicsUniversity of Fribourg CH‐1700Fribourg Switzerland;</p> <p>Department of MathematicsUniversity of Fribourg CH‐1700Fribourg Switzerland;</p> <p>Microsystems Laboratory LMIS4école Polytechnique Fédérale de Lausanne (EPFL) Station 17Lausanne Switzerland CH‐1015;</p> <p>Department of Fundamental MicrobiologyUniversity of LausanneLausanne Switzerland 1015;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> 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