Genome characteristics and environmental distribution of the first phage that infects the LD28 clade, a?freshwater methylotrophic bacterial group

Moon Kira; Kang Ilnam; Kim Suhyun; Kim Sang‐Jong; Cho Jang‐Cheon

摘要

Summary >Bacteriophages infecting major groups of freshwater heterotrophic bacteria have been rarely isolated, hampering analyses of freshwater viromes. Here, we report the isolation and genomic characterization of P19250A, the first phage that infects the LD28 clade, an abundant freshwater methylotrophic bacterial group. P19250A was isolated from Lake Soyang, an oligotrophic reservoir, using an LD28 strain as a host. Morphological and genomic analyses revealed that P19250A is a lytic siphovirus with a ～38.6‐kb genome. To analyze the distribution of P19250A genome within its habitat, six seasonal viral metagenome (virome) samples were prepared from Lake Soyang. Through binning analysis of freshwater viromes, P19250A was shown to be the most highly assigned freshwater phage that infects heterotrophic bacteria (up to 8.21%) in five viromes. Furthermore, when freshwater virome data collected worldwide were analyzed, P19250A genome also showed high abundance, especially in Lough Neagh, UK, where P19250A genome was recorded as the most abundant bacteriophage. From metagenome analysis, the proportion of P19250A‐assigned reads showed seasonal fluctuation following the abundance of the LD28 clade in Lake Soyang. These results showed that P19250A would be an essential resource for analyses of freshwater viromes, and also suggest that phages of other abundant freshwater bacteria need to be isolated for better understanding of freshwater viruses. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>摘要</ title> >噬菌体感染淡水杂种体细菌的主要群体已经很少被隔离，妨碍淡水病毒缺陷的分析。这里，我们报告了P19250A的分离和基因组特征，其感染LD28疏水的第一噬菌体，一种丰富的淡水甲型培养基细菌组。 P19250A使用LD28菌株作为宿主从苏丹湖中分离出来。形态学和基因组分析表明，P19250A是具有〜38.6-kB基因组的裂解性腹股沟病毒。为了分析其栖息地内P19250A基因组的分布，从苏丹湖制备六种季节性病毒梅毒群（病毒体）样品。通过对淡水病毒的分析，P19250A被证明是最高度分配的淡水噬菌体，其在五种病毒素中感染异养细菌（高达8.21％）。此外，当分析全球收集的淡水病毒数据时，P19250A G19250A基因组也表现出高度丰富，特别是在英国的Lough Neagh中，其中P19250A基因组被记录为最丰富的噬菌体。从Metagenome分析中，P19250A分配的读数的比例显示出苏丹湖LD28思潮丰富的季节性波动。这些结果表明，P19250A将是分析淡水病毒的基本资源，并表明其他丰富的淡水细菌的噬菌体需要孤立，以便更好地了解淡水病毒。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" > 著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology 》</a> <b style="margin: 0 2px;">|</b><span>2017年第11期</span><b style="margin: 0 2px;">|</b> <span>共14页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Moon Kira&option=202" target="_blank" rel="nofollow">Moon Kira;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kang Ilnam&option=202" target="_blank" rel="nofollow">Kang Ilnam;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kim Suhyun&option=202" target="_blank" rel="nofollow">Kim Suhyun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kim Sang‐Jong&option=202" target="_blank" rel="nofollow">Kim Sang‐Jong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cho Jang‐Cheon&option=202" target="_blank" rel="nofollow">Cho Jang‐Cheon;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>School of Biological SciencesSeoul National UniversitySeoul 08826 Republic of Korea;</p> <p>Department of Biological SciencesInha UniversityIncheon 22212 Republic of Korea;</p> <p>Department of Biological SciencesInha UniversityIncheon 22212 Republic of Korea;</p> <p>School of Biological SciencesSeoul National UniversitySeoul 08826 Republic of Korea;</p> <p>Department of Biological SciencesInha UniversityIncheon 22212 Republic of Korea;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li> <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span> <a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学 ;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" 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target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国微生态学杂志 </a> </span> <span> . 2003</span><span> ,第3期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-10891_thesis/02022229567.html">儿童系统性红斑狼疮临床特征和基因组DNA甲基化水平的研究</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=吴凤岐&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 吴凤岐</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张霆&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张霆</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=权力&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,权力</a> <span> <a href="/conference-cn-10891/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2011年北京医学会儿科学分会学术年会 </a> <span> <span> . 2011</span> </span> </div> </li> <li> <div> <b>7. </b><a 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