Two forms of phosphomannomutase in gammaproteobacteria: The overlooked membrane‐bound form of AlgC is required for twitching motility of        <i >Lysobacter enzymogenesLysobacter enzymogenes

Qian Guoliang; Fei Shifang; Galperin Michael Y.

摘要

Summary > Lysobacter enzymogenes , a member of Xanthomonadaceae , is a promising tool to control crop‐destroying fungal pathogens. One of its key antifungal virulence factors is the type IV pili that are required for twitching motility. Transposon mutagenesis of L. enzymogenes revealed that the production of type IV pili required the presence of the Le2152 gene, which encodes an AlgC‐type phosphomannomutase/phosphoglucomutase (PMM). However, in addition to the cytoplasmic PMM domain, the Le2152 gene product contains a ~200‐aa N‐terminal periplasmic domain that is anchored in the membrane by two transmembrane segments and belongs to the dCache superfamily of periplasmic sensor domains. Sequence analysis identified similar membrane‐anchored PMMs, encoded in conserved coaBC ‐ dut ‐ algC gene clusters, in a variety of gammaproteobacteria, either as the sole PMM gene in the entire genome or in addition to the gene encoding the stand‐alone enzymatic domain. Previously overlooked N‐terminal periplasmic sensor domains were detected in the well‐characterized PMMs of <fi>Pseudomonas aeruginosa</fi> and Xanthomonas campestris , albeit not in the enzymes from Pseudomonas fluorescens, Pseudomonas putida or Azotobacter vinelandii . It appears that after the initial cloning of the enzymatically active soluble part of <fi>P. aeruginosa</fi> AlgC in 1991, all subsequent studies utilized N‐terminally truncated open reading frames. The N‐terminal dCache sensor domain of AlgC is predicted to modulate the PMM activity of the cytoplasmic domain in response to as yet unidentified environmental signal(s). AlgC‐like m </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>概述</ title> > 粘滞符酶</ i>， xanthomonadaceae的成员</ i>，是一种有助于控制作物破坏的真菌病原体的工具。其关键的抗真菌毒力因子之一是抽搐运动所需的IV型Pili。转座子诱变 l。酶</ i>揭示了IV型pili的产生需要，所以编码藻类型磷酰胺酶/磷酸葡糖酶（PMM）的含量。然而，除了细胞质PMM结构域之外，LE2152基因产物含有〜200-AA N-末端周质结构域，其通过两个跨膜段锚定，并属于周质传感器结构域的DCACH超家族。序列分析鉴定了类似的膜锚定PMM，在保守的中编码 COABC </ i> - <I> DUT </ i> - 藻类</ i>基因簇，在各种伽血曲霉菌中，如在整个基因组中唯一PMM基因，或者除了编码独立酶促结构域的基因之外。以前被忽视的N-终端周质传感器结构域在<fi>假单胞菌铜绿假单胞菌的良好表征PMMS中检测到 xanthomonas campestris </ i>，尽管来自来自假单胞菌的酶，假单胞菌荧光Pivida </ i>或 Azotobacter vinelandii </ i>。似乎在初始克隆<fi> p的酶活性可溶性部分之后。 Acuginosa </ Fi> algc在1991年，所有后续研究都利用了n个末端截断的开放阅读框架。预计恒定的N末端DCache传感器结构域响应于尚未识别的环境信号调节细胞质结构域的PMM活性。 allc样m </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22868/'>《Environmental microbiology》</a> <b style="margin: 0 2px;">|</b><span>2019年第11期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Qian Guoliang&option=202" target="_blank" rel="nofollow">Qian Guoliang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fei Shifang&option=202" target="_blank" rel="nofollow">Fei Shifang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Galperin Michael Y.&option=202" target="_blank" rel="nofollow">Galperin Michael Y.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>College of Plant ProtectionNanjing Agricultural UniversityNanjing 210095 China;</p> <p>College of Plant ProtectionNanjing Agricultural UniversityNanjing 210095 China;</p> <p>National Center for Biotechnology Information National Library of MedicineNational Institutes of HealthBethesda MD 20894 USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023228459.html">Two forms of phosphomannomutase in gammaproteobacteria: The overlooked membrane‐bound form of AlgC is required for twitching motility of <i >Lysobacter enzymogenesLysobacter enzymogenes</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Qian Guoliang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Qian Guoliang,</a> <a 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href="/academic-conference-cn_meeting-12830_thesis/02022743650.html">嗜水气单胞菌TPS-30株丝氨酸蛋白酶基因与溶血素基因在大肠杆菌中的融合表达</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=潘晓艺&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 潘晓艺</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=沈锦玉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,沈锦玉</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=郝贵杰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,郝贵杰</a> <span> <a href="/conference-cn-12830/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第十二届中国科协年会 </a> <span> <span> . 2010</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020314504103.html">一株诱变枯草杆菌溶血栓酶的分离纯化及性质研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=祁晖&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 祁晖</a> <span> . 2003</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061204544670.html">一种表达甲酰胺酶与亚磷酸脱氢酶融合蛋白的重组大肠杆菌及其构建方法与应用</a> <b>[P]</b> . <span> 中国专利： CN108315288B </span> <span> . 2021.07.20</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120110797975.html">一种表达甲酰胺酶与亚磷酸脱氢酶融合蛋白的重组大肠杆菌及其构建方法与应用</a> <b>[P]</b> . <span> 中国专利： CN108315288A </span> <span> . 2018-07-24</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130421993096.html">METHOD AND DEVICE FOR FORMING FLEXIBLE MEMBRANE CAPABLE OF REDUCING REQUIRED TIME FROM A MEMBRANE DRY PROCESS TO SEPARATION PROCESS THROUGH A MEMBRANE COOLING PROCESS, AND A METHOD FOR FORMING SOLUTION FILM</a> <b>[P]</b> . <span> 外国专利：  KR20120107850A </span> <span> . 2012-10-04</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过膜冷却过程形成可缩短从膜干燥过程到分离过程所需时间的柔性膜的方法和装置，以及形成溶液膜的方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130459741649.html">Thermoforming synthetic parts with complex curved surfaces partic. transparent polycarbonate for automobile use - by placing blank between flexible gas tight membrane and former of required shape made from rigid porous body coated with gas permeable surface and drawing vacuum through the porous body causing membrane</a> <b>[P]</b> . <span> 外国专利：  FR2696677A1 </span> <span> . 1994-04-15</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> 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Two forms of phosphomannomutase in gammaproteobacteria: The overlooked membrane‐bound form of AlgC is required for twitching motility of Lysobacter enzymogenesLysobacter enzymogenes

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