Implementation of a Simple Nanostructured Bio‐electrode with Immobilized <i xmlns='http://www.wiley.com/namespaces/wiley'>Rhus VerniciferaRhus Vernicifera Laccase for Oxygen Sensing Applications

Torrinha álvaro; Montenegro Maria C. B. S. M.; Araújo Alberto N.

摘要

Abstract > In this work a simple nanostructured direct‐electron transfer bio‐electrode based on tree laccase from Rhus vernicifera is described. The electrode was implemented on a 2?mm diameter graphite mine casted with a reduced graphene surface presenting the specific capacitance of 195.8 F g ?1 . About 10 μl of mixture between 25?mg mL ?1 laccase suspension and 5?mg mL ?1 single‐walled carbon nanotubes in 2?% SDS is dropped over the surface followed by 5 μl of the biological friendly tetrakis(2,3‐dihydroxypropyl)‐silane monomer sol to provide physical entrapment in a silica matrix after gelation. The rigidity of enzyme encapsulation allowed to obtain a constant enzyme turnover of about 16?min ?1 in the extended pH range of 6.0‐7.5, being the activity almost proportional to the temperature used in the interval between 25 and 40 °C. The graphite‐graphene/SWCNT‐laccase/sol‐gel electrode enabled a proportional response to molecular oxygen up to the concentration of 0.45?mmol L ?1 and is capable to generate the maximum power of 4.5 μW cm ?2 at 0.250?V vs the AgCl/Ag reference electrode in quiescent oxygen saturated solution. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >基于从 Rhus vernicifera </ i>的树漆酶工作，工作简单的纳米结构直接电子转移生物电极。电极在铸造的2×mm直径的石墨矿上实施，其具有呈现195.8fg α1/ sup>的比电容的特定电容。约10μl混合物在25μmgmlα1-1-</ sup>漆壳悬浮液中，5·mg ml oth 2→单壁碳纳米管中的2·％SDS在表面上滴下其次是5μL生物友好的四（2,3-二羟基丙基） - 硅烷单体溶胶，在凝胶化后在二氧化硅基质中提供物理夹带。酶包封的刚性允许在延长的pH范围为6.0-7.5的延长pH范围内获得约16Ω·5的恒定酶转换，是与25之间的间隔中使用的温度几乎成比例的活动和40°C。石墨烯/ SWCNT-LACQUAS /溶胶 - 凝胶电极使分子氧的比例响应至0.45Ω·莫醇L <SUP>α1</ sup>并且能够产生4.5μwcm的最大功率 2 </ sup>在0.250〜Vs静态氧饱和溶液中的AGCl / Ag参考电极。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2017年第6期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Torrinha álvaro&option=202" target="_blank" rel="nofollow">Torrinha álvaro;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Montenegro Maria C. B. S. M.&option=202" target="_blank" rel="nofollow">Montenegro Maria C. B. S. M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Araújo Alberto N.&option=202" target="_blank" rel="nofollow">Araújo Alberto N.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>LAQV-REQUIMTE Lab. Química AplicadaFac. Farmácia (U.P.)Porto Portugal;</p> <p>LAQV-REQUIMTE Lab. Química AplicadaFac. Farmácia (U.P.)Porto Portugal;</p> <p>LAQV-REQUIMTE Lab. Química AplicadaFac. Farmácia (U.P.)Porto Portugal;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Biosensors&option=203" rel="nofollow">Biosensors;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=direct electron transfer&option=203" rel="nofollow">direct electron transfer;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=laccase from Rhus vernicifera&option=203" rel="nofollow">laccase from Rhus vernicifera;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=nanostructured electrode&option=203" rel="nofollow">nanostructured electrode;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=sol-gel&option=203" rel="nofollow">sol-gel;</a> </p> <div class="translation"> 机译：生物传感器;直接电子转移;来自Rhus Vernicifera的漆酶;纳米结构;溶胶 - 凝胶; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023174701.html">Implementation of a Simple Nanostructured Bio‐electrode with Immobilized <i xmlns="http://www.wiley.com/namespaces/wiley">Rhus VerniciferaRhus Vernicifera Laccase for Oxygen Sensing Applications</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Torrinha álvaro&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Torrinha álvaro,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Montenegro Maria C. 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Implementation of a Simple Nanostructured Bio‐electrode with Immobilized Rhus VerniciferaRhus Vernicifera Laccase for Oxygen Sensing Applications

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