Comparative appraisal of HPLC, Chloramine-T and Lane-Eynon methods for quantification of carbohydrates in concentrated dairy products

摘要

Lactose and sucrose were quantified in sweetened condensed milk and 'Dulce de leche' using a high-performance liquid chromatography (HPLC) approach as compared with the classical methods (Chloramine-T and Lane-Eynon). Lane-Eynon method estimated the lowest values of carbohydrates for both products. The chromatographic method quantified the carbohydrate contents at the same range as Chloramine-T method. The values of the estimated standard error (ESE) for HPLC versus Chloramine-T (0.55-0.77 mass %) were lower than the ESE for HPLC versus Lane-Eynon (1.01-1.63 mass %). Calcium concentration in the products may explain underestimated carbohydrate contents by the Lane-Eynon method.