The effect of molting hormone (20-hydroxyecdyson) on molting of mud crab (Scylla olivacea Herbst, 1976)

摘要

Molting in crustaceans is influenced by molting hormone. This study aims to examine the effect of adding the molting hormone 20- hydroxyecdyson (20-HE) against the concentration of ecdysteroid haemolymph and molting of mud crab. The study is designedusing Complete Randomized Design (CRD), consisting of four dose treatments of 20-HE, namely: (A) 0 ug/mL (without hormone), (B) 1.0 ug/mL, (C) 1.3 ug/mL and (D) 1.6 ug/mL; each treatment is repeated three times. The addition of molting hormone 20-HE is done by injection. Parameters measured are ecdysteroid haemolymph concentration post-injection, the percentage of molting, molting duration, weight and width of carapace, survival rate, as well as water quality as the growth medium. Analysis of the sampleof molting hormone and haemolymph is performed using Ultra-Fast Liquid Chromatography (UFLC). Concentration of ecdysteroid haemolymph pre and post injection of vitomolt is performed by t-test. To determine the effect of molting treatment on mangrove crab, analysis of variance (ANOVA) is done. The results show that the addition of 20-HE molting hormone increases the concentration of molting hormone (ecdysteroid haemolymph), increases the percentage of molting, accelerates molting duration, increases theweight and width of the carapace, and increases survival of mangrove crabs. The higher the dose of molting hormone injected, the higher the concentration of ecdysteroid haemolymph post-injection. The 20-HE hormone dose of 1.0 ug/mL results in the highest percentage of molting, fastest molting duration, the highest increase of weight and width of carapace, and the highest survival rate.