Co-cultivation of Unicellular Green Algae (Chlorophyta, Chlorophyceae) and Lymphocytes of Peripheral Blood of Humans as a Test System for Radiobiological Studies

摘要

The study proposes a new model lest system for fundamental radiobiological studies (in particular, for "bystander effect" research). It is based on the co-cultivation of phylogenetically distant human peripheral blood lymphocytes and unicellular greenalgae. The test system allows reducing the number of analyzed factors by limiting the types of intercellular signaling from test object 2 as the probable donor of the damaging signal (green alga) to test object 1 (human peripheral blood lymphocytes). Itwas found that variants of the test system, wherein the second test objects are represented by different species of unicellular green algae {Chlamydomonas moewusii Gerloff 1940, Chlamydomonas sp. ACKU 221-03, Haematococcus pluvialis Flotow emend. Wille1903, and Ettlia carotinosa Komarek 1989), which conform to the radiobiological requirements to the two-component test system. All investigated species are compatible with the culture of lymphocytes, both in cultural conditions and the procedure of chromosomal material staining. The studied strains of algae do not cause toxic or mutagenic effect and do not affect the cell growth of lymphocytes.