Biodistribution, pharmacokinetics and radioimmunotherapy of ~188Re-cetuximab in NCI-H292 human lung tumor-bearing nude mice

摘要

Background Cetuximab is a fully humanized IgG1 subclass monoclonal that binds specifically to the human epidermal growth factor receptor (EGFR). Although EGFR is expressed in normal cells, the overexpression of EGFR is detected in many human cancers, such as colon, rectum and lung tumors. In this study, cetuximab with a combination of radiotherapy nuclear 188Re achieved better therapeutic effect on lung cancer. Methods ~188Re-cetuximab administered by the i.v. route in human NCI-H292 lung tumor-bearing mice was investigated. NanoSPECT/CT images were taken to evaluate the distribution and tumor targeting of ~188Re-cetuximab in mice. The anti-tumor effect of ~188Re-cetuximab was assessed by the tumor growth inhibition, survival ratio. Results For nanoSPECT/CT imaging, a significant uptake in tumor was observed at 24 and 48 h following the injection of ~188Re-cetuximab. The anti-tumor effect of ~188Re-cetuximab was assessed by tumor growth inhibition and the survival ratio. The tumor-bearing mice treated with ~188Re-cetuximab showed a better mean tumor growth inhibition rate (MGI = 0.049) and longer median survival time and lifespan (62.50 d; 70.07%) than those treated with ~188Re-perrhenate and cetuximab only by single injection. A synergistic effect of tumor growth inhibition was observed with the combination index exceeding one for~188Re-cetuximab (CI = 6.135 and 9.276). Conclusion The tumor targeting and localization of ~188Re-cetuximab were confirmed in this study. Synergistic therapeutic efficacy was demonstrated for the radioimmunotherapy of ~188Re-cetuximab. The results of this study reveal the potential advantage and benefit obtained from ~188Re-cetuximab for diagnosis and therapy of oncology applications in the future.