Evaluation of chromosomal aberrations induced by Re-188-dendrimer nanosystem on B16f1 melanoma cells

摘要

Purpose: To study the rhenium-188 labeling of polyamidoamine (PAMAM) generation 4 (G4) dendrimer and its evaluation on biodistribution and chromosomal aberrations in melanoma cells induced by ionizing radiation as potential treatment agent.Materials and methods: Dendrimers were first conjugated with Suc-HYNIC (succinimidyl 6-hydrazinopyridine-3-carboxylic acid hydrochloride). Dendrimer-HYNIC was then incubated with (ReO4-)-Re-188. Biodistribution was performed administrating Re-188-dendrimer to normal (NM) or melanoma-bearing mice (MBM). Chromosome aberration test was conducted in order to measure treatment capacity of Re-188-dendrimer in melanoma cells.Results: Radiolabeling yield of dendrimer was approx. 70%. Biodistribution studies in NM showed blood clearance with hepatic and renal depuration. MBM showed a similar pattern of biodistribution with tumor uptake of 6% of injected dose. Aberrant metaphases quantified in control cells were 7%, increasing to 29.5% in cells treated with 15Ci (0.555MBq) of Re-188-dendrimer for 24h.Conclusions: Re-188-dendrimer can produce double-stranded breaks in DNA induced by ionizing radiation in melanoma cells in vitro.