MALDI MS-based analysis of oligosaccharides using 4-(diphenylphosphinyl) benzenamine as non-reductive amination derivatization reagent

摘要

Analysis of native oligosaccharides by MS has been a challenge due in part to their limited ionization capacity. To circumvent the problem, oligosaccharides may be chemically derivatized to improve their ionization efficiency. Here, a phosphorus-containing aniline derivative, 4-(diphenylphosphinyl)benzeneamine (4-DPPBA) was used as a reagent for oligosaccharides non-reductive amination derivatization. In general, without the tedious purification process, a significant increase about 5-fold in the mass signal intensities and 4-fold in S/N ratio were obtained. The derivatization rate is above 98% under the optimized reaction conditions. Compared with aniline, 4-DPPBA shows some merits, such as high derivatization rate, signal intensity, S/N ratio and the changeless fragment pattern between derivative and non-derivative oligosaccharide chains. Based on the advantages, 4-DPPBA is of great potential to be one kind of oligosaccharides derivatization reagent with high performance and widely used in the analysis of oligosaccharides. (C) 2017 Elsevier B.V. All rights reserved.