Investigation of brucellosis in cattle and buffaloes by conventional and molecular assays

摘要

The present study was carried out to evaluate the conventional and molecular techniques for diagnosis of bovine brucellosis. A total of four isolates of Brucella abortus obtained from 100 clinical samples of foetal stomach contents, vaginal mucus and uterine discharges were characterized biochemically. The isolates were confirmed as Brucella spp. by PCR using B4/B5 primer pair and as B. abortus by Bruce Ladder multiplex PCR. By Hinic Real-time PCR, all the four isolates were confirmed as Brucella spp. with C-t values between 14-16. With DNA extracted from 40 clinical samples of foetal stomach contents, vaginal mucus and uterine discharges, Real time PCR appeared most sensitive of the three molecular and conventional techniques as it detected maximum number of positive samples.