A SINGLE NUCLEOTIDE EXTENSION ASSAY IDENTIFICATION AND DIFFERENTIATION OF CATTLE AND BUFFALO MEAT

摘要

This invention relates to a single nucleotide extension assay identification and differentiation of cattle and buffalo meat comprising, designing and developing Genotyping primer from cytochrome b gene, hybridizing the genotyping primer to the sample DNA immediately upstream to target SNP nucleotide, wherein DNA polymerase extend a single fluorescently labeled dideoxy nucleotide complementary to the target SNP to the 3 end of the hybridized primer, Termination of the extension after the incorporation of single dd NTP, wherein missing 3-hydroxyl in dideoxy nucleotide prevents further nucleotides from being added.