Antibacterial Activity and Cytotoxicity of Co/Fe Doped ZnO Nanoparticles

摘要

The Co/Fe doped and (Co, Fe) co-doped ZnO nanoparticles of composition Zn_(0.85)Co_(0.15-x)Fe_xO (where x = 0, 0.15,0.10, 0.05 labelled as ZC, ZF, ZCF10, ZCF5) have been prepared by mechanochemical method using ZnCl_2, CoCl_2 .6H_2O, FeC_2O_4 -2H_2O and Na_2C_2O_4 as starting materials and characterized by X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). XRD results showed that Co/Fe doped and (Co, Fe) co-doped ZnO nanoparticles possess hexagonal wurtzite crystal structure with good crystallinity having average crystallite size calculated from Debye-Scherrer's equation in 17-32 nm range. The absorption bands observed in 478-445 cm~(-1) region due to v_(Zn-O) mode indicated that Co and Fe have substituted Zn in ZnO matrix. SEM images revealed that Co doped ZnO nanocrystalline powder (ZC) possesses spherical structure whereas Fe doped (ZF) and (Co, Fe) co-doped (ZCF10 and ZCF5) samples have shown hexagonal structures. The antibacterial activity of the prepared samples assayed against Gram +ve bacteria Staphylococcus aureus and Bacillus cereus and Gram -ve bacteria Salmonella typhi and Escherichia coli by MIC method has shown ZC and co-doped ZCF10 and ZCF5 nanoparticles constitute promising antibacterial agents compared to ZF. The in vitro cytotoxicity of the samples studied on mammalian transformed cell line Hep2C, a derivative of human cervix carcinoma HeLa cells by MTT assay has shown that among four samples ZCF5 exhibits highest cytotoxicity.