Production of IgY Against Infectious Bursal Disease Virus and Purification of IgY from Egg by Using Biocompatible Technique

摘要

Infectious bursal disease (IBD) virus is a highly contagious pathogen that causes damage to lymphoid organs and remains a threat to the poultry industry worldwide. IBD virus infection causes immunosuppression that leads to increased susceptibility to secondary infection and failed of vaccination programs. Specific IgY against IBDV isolated from egg yolk could be applied as passive immunization to susceptible young bird in the susceptible period (19-26 days). The aim of this study was to produce immunoglobulin Y against IBD virus in layer chicken and to purify IgY from egg yolk by using biocompatible reagent. Ten female, Isa Brown layer chickens were vaccinated with IBD vaccine. The biocompatible technique to purify of IgY from egg yolks in this study consists of two main procedures, namely lipid removal from eggs yolk and IgY precipitation from the supernatant of the first step. Separation of lipids from egg yolk was done by a combination of water dilution, freezing, and centrifugation method. Isolation of IgY was done by salting out using 8.8% NaCl at pH 4.0. This technique was able to produce high purify IgY without decreased in immunoactivity of IgY. This technique is environmentally friendly and simple for IgY purification.