Construction of a Chimeric Plasmid Vector and Its Investigation for Usage as an Internal Control for Detection of Shiga Toxin-Producing Escherichia coli by Polymerase Chain Reaction and Real-Time PCR

摘要

Background: Shiga-toxigenic Escherichia coli (STEC) is an important foodborne pathogen and considered a worldwide problem. The infection can cause severe illnesses, including hemolytic uremic syndrome. Traditional methods for STEC detection are currently not done in clinical laboratories due to the cost and related long turnaround time, which necessitates the development of quick and reliable alternative detection methods.