Detection of 16Sr XI group of phytoplasma in non-symptomatic sugarcane cultivars in Eastern Uttar Pradesh, India

摘要

Twenty six non-symptomatic leaf samples from six different sugarcane varieties (CoS 07250, CoS 96268, CoS 91269, CoSe 01424, CoS 08272, CoS 08279) grown at Sugarcane Research Institute farm at Shahjahanpur were analyzed for phytoplasma presence by using P1/P6 and R16F2n/R2 primer pairs.The amplification product of 1.2 kb was obtained in 17 out of the 26 non-symptomatic leaf samples in nested PCR assays.The amplified products from 17 positive samples were directly sequenced, aligned and analyzed usingBLASTn. The obtained 16SrRNA gene sequences of all the 17 non-symptomatic sugarcane phytoplasma isolates were found identical (99-100%) among themselves and showed 99% sequence identity to member strains of a 16SrXI group of phytoplasma. The informationof phytoplasma association with non-symptomatic sugarcane varieties at early stage is important and needs immediate attention because at crop maturity the disease may become serious and responsible for major losses to yield and sugar recovery.