Synthesis of reversible nucleoside amino acid conjugates

摘要

A general synthetic method to conjugate cysteine-containing peptides to nucleosides via a disulfide link has been developed, using the heterobifunctional reagent N-succinimidyl-3-(2-pyridylthio)propionate(SPDP0 and amino-modified nucleosides. Representative compounds based on C-6 modified adenosine, C-5 modified uridine and 2'-deoxyuridine are reported, along with extensive NMR characterizations of these novel nucleosides.