Detection of Enzymes and Proteins Produced From Some Algae Isolated from Iraqi Aquatic Environment

摘要

The research was carried out to detection of enzymes (protease, amylase, beta-alkaline, phosphatase, and alkaline phosphatase) and proteins, detect two enzymes (protease, amylase) and proteins in ten local algae isolates, nine that belong to the blue-green algae and one to the green algae. The algae isolated from Tigris River in Iraq have been developedin BG-11 enrichment culture media. The result nine isolated algae (Anabaena variabilia, Lyngbya digueti, Lyngbya limnetic, Microcystis aeruginosa, Oscillatoria limosa, Oscillatoria sancta, Phormidium mucicola, Spirulina laxissima, Westiellopsis prolific) had the capability to produce the enzymes protease and amylase. The efficiency of the protease enzyme for green blue algae was 10.115-13.3 u ml"1 andthe amylase enzyme activity was 0.112- 0.562 u ml"1, either the algae Chrococcum sp. The yield of the Green Algae Division was as high as the protease and amylase activity was 3.7 and 0.374 u ml"1, respectively).The result indicated the capability to produce the proteins from the 10 isolates after development within a biomass ranged from 0.0607-0.5380 mg I"1 and protein content of 2.280-9.950 mg I"1 for algal. The Chrococcum sp. that belong to Green Algae produced protease, amylase, and betaalactase with concentrations of 3.7, 0.374 and 26.7 u ml"1, respectively, and its production capability was 4.210 mg I"1. The productivity of the phosphatase enzyme was0.06 and 0.03 u ml"1 Anabaena variabilia and Lyngbya digueti respectively, while the alklin phosphates enzyme was not produced in all studied algal isolates.