Development and Validation of UV Spectrophotometric Method for Estimation of Cyproheptadine hydrochloride

摘要

A new, simple, accurate and sensitive UV -spectrophotometric absorption correction method has been developed for estimation of cyproheptadine hydrochloride in bulk utilizing concept of standard addition. The method is based upon determination of cyproheptadine hydrochloride at 286nm, distilled water as a solvent. Linearity was observed in range of 2-10ug/ml for cyproheptadine hydrochloride. The correction coefficient value was found to near tol. All validation methods were validated as per ICH guideline.