Combining the Sclerotinia stem rot disease resistance trait in interspecific hybrids of Brassica napus and Brassica carinata

摘要

The present study involved one line from each Brassica carinata, an allotetraploid with B and C genomes (BBCC, 2n = 34) 'Bcarl 15' and Brassica napus, an allotetraploid with A and C genomes (AACC, 2n = 38) 'Bnapl 14', identified as resistant to Sclerotinia stem rot disease, to cross reciprocally for congregating the trait of stem rot disease resistance in Fr The crossability between these two species was confirmed by morphology and simple sequence repeat (SSR) analysis. The hybrids between these twospecies were obtained normally but the fertility in F: plant remained intact only when B. carinata was used as the female parent. The hybrid plants exhibited intermediate leaf, stem, inflorescence and flower morphology and were found resistant to the Sclerotinia stem rot disease resistance. One plant of B. carinata x B. napus and ten plants of B. napus * B. carinata obtained were exhibited 2% and 100% pollen sterility, respectively. Hence, seed setting through self-pollination were only observed in Fl plant where Bcarl 15 was used in the female form. The in vivo stem inoculation assay showed that hybrid plants were inheriting resistance for the Sclerotinia stem rot disease from both of its parents. Molecular analysis of F hybrids indicated that traitsof economic importance like disease resistance could be transferred in either case of reciprocal crosses between B. carinata and B. napus through interspecific hybridization.