Jiedu Huoxue decoction improves inflammation in rat type III prostatitis: The importance of the NF-kappa B signalling pathway

摘要

This study aimed to investigate the mechanism of Jiedu Huoxue decoction (JDHXD) in type III prostatitis based on the NF-kappa B signalling pathway. Twenty-six SpragueDawley male rats were divided into blank control, model, positive (Prostate Plus), low-dose JDHXD, medium-dose JDHXD and high-dose JDHXD groups. Type III prostatitis rat model was established and confirmed with HE staining. NF-kappa B P50 and NF-kappa B P65 expression was detected with immunohistochemistry. NF-kappa B mRNA expression was detected with qRT-PCR. Protein expression of NF-kappa B and its inhibitor I kappa-Ba was detected with Western blot. Compared to the model group, a decrease in glandular hyperplasia and inflammation, and in NF-kappa B P50 and NF-kappa B P65 expression in the medium-and high-dose JDHXD groups was observed. NF-kappa B mRNA expression was significantly increased in the model group compared to control (p < 0.05), and significantly decreased in the JDHXD treatment groups compared to model group (p < 0.05). Protein expression of NF-kappa B was significantly increased in the model and low-dose JDHXD groups compared to control(p < 0.05), and significantly decreased in the medium-and high-dose JDHXD groups compared to model group (p < 0.05). Protein expression of I kappa-Ba was vice versa. JDHXD could be a potential treatment for type III prostatitis via its regulation of NF-kappa B and I kappa-Ba expression.