Improving the quality of cryopreserved goat semen with a commercial bull extender supplemented with resveratrol

摘要

The purpose of the current study was to evaluate the effects of resveratrol (RSV) on the quality of frozen-thawed goat sperm. Semen samples from four bucks were divided into five aliquots and diluted with a commercial bull semen extender containing: no antioxidant (RSV-0, control), 10 mu M RSV (RSV-10), 50 mu M RSV (RSV-50), 100 mu M RSV (RSV-100) and 250 mu M RSV (RSV-250). After thawing, sperm motility, abnormal morphology, membrane and acrosome integrity, mitochondrial activity, phosphatidylserine (PS) distribution, and oxidative stress were evaluated. The results indicated that in comparison with the control, when the concentration of RSV was 10 or 50 mu M, the total motility, progressive motility, membrane and acrosome integrity, and mitochondrial activity of post-thaw spermatozoa was greater (P 0.05). Additionally, the use of extenders containing RSV-10 or RSV-50 resulted in a greater percentage of viable spermatozoa as compared to the other groups (P 0.05). Importantly, there were more viable spermatozoa (49.61 +/- 0.61%) and less non-viable spermatozoa (49.16 +/- 1.01%) in the RSV-50 group compared to the other extenders (P 0.05). Furthermore, the use of the extenders containing RSV-10 and -50 resulted in a reduction in ROS production in frozen-thawed spermatozoa as compared to the control (P 0.05). There, however, was no difference among extenders for abnormal morphology and PS distribution. In conclusion, supplementation with RSV, at a concentration of 10 or 50 mu M in the semen extender, can improve the post-thaw goat sperm quality, which may occur as a consequence of inhibition of ROS generation.