Effects in broiler hens of genetic lines differing in fertility, biotin supplementation, and age on relative abundance of oviductal transforming growth factor-beta and carbonic anhydrase mRNA transcripts

摘要

There was evaluation of effects of biotin administration on oviductal abundance of transforming growth factor-beta (TGF-beta) and carbonic anhydrase (CA) mRNA transcript in younger and older broiler hens of relatively lesser and greater fertility lines. Additionally, effects of biotin supplementation on attenuation of age-related subfertility were evaluated. Hens from the relatively greater (Line D, n = 60) and lesser (Line B, n = 60) fertility rate line were randomly assigned to three treatment groups. Biotin was not or was administered in drinking water from 30 to 33 (younger age) and 53 to 56 (older age) wk of age to have access to no biotin (T-0), or 0.3 (T-1), or 0.45 (T-2) mg/L of biotin. There was assessment the relative oviductal abundances of TGF-beta and CA mRNA transcript abundances. Supplemental biotin and age had no effect on the relative abundance of oviductal TGF-beta mRNA transcript in hens of Line D. There, however, was a ten-fold greater abundance of TGF-beta in hens of the T-0 group of Line B compared with Line D. Relative abundance of TGF-beta mRNA transcript was greater in younger hens of Line B; however, biotin supplementation of older hens of the T-2 group of Line B resulted in a similar TGF-beta abundance to that of younger hens. Inconstant with the TGF-beta abundance, CA abundance in hens of Line B was not affected by supplemental biotin or bird age. Overall, differences in TGF-beta or CA abundances did not affect fertility of broiler hens.