Garcinia mangostana pericarp extract protects against oxidative stress and cardiovascular remodeling via suppression of p47(phox) and iNOS in nitric oxide deficient rats

摘要

N-omega-Nitro-L-arginine methyl ester (L-NAME)-induced hypertension and cardiovascular remodeling are associated with oxidative stress and inflammation. Garcinia mangostana Linn., has been reported to have antioxidant and anti-inflammatory properties. This study investigated whether G. mangostana pericarp extract (GME) could prevent L-NAME -induced hemodynamic alterations, cardiovascular remodeling, oxidative stress and inflammation in rats. Male Sprague-Dawley rats were given 40 mg/kg/day of L-NAME in drinking water to induce hypertension, and were simultaneously treated with GME at a dose of 200 mg/kg/day. Rats that received L-NAME for five weeks had high blood pressure, left ventricular hypertrophy and thickening of aortic wall. Vascular superoxide production, plasma malondialdehyde (MDA), and plasma tumor necrosis factor alpha (TNF-alpha)were significantly increased in L-NAME-hypertensive rats (p < 0.05). This was consistent with up-regulation of the p47(phox) NADPH oxidase subunit and iNOS protein expression in aortic tissues (p < 0.05). Low levels of plasma nitric oxide metabolites were observed in L-NAME hypertension. GME prevented the development of hypertension and cardiovascular remodeling induced by L-NAME with reduced oxidative stress and inflammation. These data suggest that GME had a protective effect against L-NAME-induced hypertension and cardiovascular remodeling via suppressing p47(phox) NADPH oxidase subunit and iNOS protein expression resulting in enhancing NO bioavailability. (C) 2017 Elsevier GmbH. All rights reserved.