Emulation of an ELISA reader and quantitative image analysis using thecomputer program Digital-OD

摘要

Here, we present a Microsoft(R) Windows(R) application that has been written to emulate a microplate reader. After scanning a standard 96-well microplate, color gradations in the slots can be analyzed quantitatively and be used for the estimation of enzyme kinetics, binding assays or concentration determination. In a test, series accuracy of the program was determined We show that the returned optical densities correlated highly with the true concentrations (r >0.97). Thus, the software is useful for a wide number of applications. Further the program is easy to handle and is available free of charge.