Biocontrol of soil diseases and soil profile associated with rhizosphere of rice (Oryza sativaSubsp.Japonica) growing paddy fields in Kansai region, Japan

摘要

This research focused onin vitroandin vivobiocontrol of two major rice diseases, Bacterial Leaf Blight caused byXanthomonus oryzae pv oryzae(Xoo) and Sheath Blight caused byRhizoctonia solani. Furthermore, the soil of paddy fields in Kansai region, Japan was analysed for the presence of these pathogens, and their microbial communities were evaluated according to the location and management systems. For biocontrol, the rhizospheric bacteria were isolated and molecularly identified. The results indicated that DAR17225040 and DAR17225017 were 99% similar toBacillus aryabhattaiandBacillus megaterium. The isolated bacteria were able to suppress Bacterial Leaf Blight and Sheath Blight disease 78% and 86% respectively, under greenhouse conditions. The 16S amplicon sequencing (Illumina MiSeq 250bp) was used to analyse the bacterial community in paddy fields of soil taken from five locations under conventional and organic systems.XooandR. solaniwere detected in sampled soil, however, no clear difference was noted, regarding systems. From amplicon sequencing data,Chloroflexi, Acidobacteria, Proteobacteria, Actinobacteria, Planctomyceteswere the dominant phyla of rice soil in Kansai region. The phylumKazan-3B-28was found more in organic system than conventional. The bacterial community was more affected by location and soil type than by management systems.