Cloning, expression and purification of SmpB from Mycobacterium tuberculosis.

Kovacs L; Megyeri K; Juhasz A; Zaja A; Miczak A

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Cloning, expression and purification of SmpB from Mycobacterium tuberculosis.

机译：结核分枝杆菌SmpB的克隆，表达和纯化。

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SmpB, a small tmRNA binding protein, is essential for trans-translation. 6His and FLAG tagged SmpB was cloned from Mycobacterium tuberculosis H37Rv. It was expressed in Escherichia coli using the T7 promoter-polymerase system. Anti-FLAG M2 agarose was used for its purification. Mycobacterial SmpB copurifies with other proteins. We identified elongation factor EF-Tu in the purified SmpB preparations.

机译：SmpB是一种小的tmRNA结合蛋白，对于反式翻译至关重要。从结核分枝杆菌H37Rv克隆了6His和FLAG标记的SmpB。使用T7启动子-聚合酶系统在大肠杆菌中表达。抗FLAG M2琼脂糖用于其纯化。分枝杆菌SmpB与其他蛋白质共纯化。我们在纯化的SmpB制剂中鉴定了延伸因子EF-Tu。

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《Acta microbiologica et immunologica Hungarica: A quarterly of the Hungarian Academy of Sciences》 |2004年第3期|共6页
作者
Kovacs L; Megyeri K; Juhasz A; Zaja A; Miczak A;
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正文语种 eng
中图分类基础医学;
关键词
purification; Mycobacterium tuberculosis; Cloning; m2 lt; 2gt; T7; 分支杆菌; 结核;

机译：purification;Mycobacterium tuberculosis;Cloning;m2 2;T7;分支杆菌;结核;

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1. Cloning, expression and purification of SmpB from Mycobacterium tuberculosis. [J] . Kovacs L, Megyeri K, Juhasz A, Acta microbiologica et immunologica Hungarica: A quarterly of the Hungarian Academy of Sciences . 2004,第3期

机译：结核分枝杆菌SmpB的克隆，表达和纯化。
2. Expression, purification and characterization of the full-length SmpB protein from Mycobacterium tuberculosis [J] . Yang Juanjuan, Liu Yindi, Xu Shuli, Protein Expression and Purification . 2018,第期

机译：结核分枝杆菌全长SMPB蛋白的表达，纯化和表征
3. Molecular cloning, purification, and serological characterization of MPT63, a novel antigen secreted by Mycobacterium tuberculosis. [J] . C Manca, K Lyashchenko, H G Wiker, Infection and immunity . 1997,第1期

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4. Molecular Cloning and Cold Shock Induced Overexpression of The DNA Encoding PhoR Sensor Domain from Mycobacterium tuberculosis as A Target Molecule for Novel Anti-Tubercular Drugs [C] . Gladys Emmanuella Putri Langi, Maelita R. Moeis, Ihsanawati, ICMNS 2012 . 2014

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5. Modeling of metabolism and gene expression under hypoxia in Mycobacterium tuberculosis. [D] . Elghraoui, Afif. 2015

机译：低氧条件下结核分枝杆菌的代谢和基因表达模型。
6. Cloning expression purification crystallization and preliminary X-ray diffraction analysis of DapA (Rv2753c) from Mycobacterium tuberculosis. Corrigendum [O] . Georgia Kefala, Manfred S. Weiss 2008

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7. Cloning, expression, purification and crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 from Mycobacterium tuberculosis. [O] . Hatzopoulos, G. N., Müller-Dieckmann, J. 2007

机译：结核分枝杆菌起始因子1的克隆，表达，纯化和结晶以及初步X射线晶体学分析。

Cloning, expression and purification of SmpB from Mycobacterium tuberculosis.

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