Whey Proteins Separation: Separation of Whey Proteins Using Modified Cellulose Fibre: An Economical Approach

摘要

Background: Whey is a watery part (serum) that remains after the coagulation of milk by acid or proteolyticenzymes and it contains mainly, lactose, proteins, minerals and water. Most of the countries havelarge dairy and cheese production units which generate huge amounts of whey per liter of milk processed.However, land disposal of whey as a waste product has been widely practiced all over the worldthat poses serious pollution problems. Thus, it is highly essential that whey has to be processed furtherto generate more valuable products and subsequently to make it suitable for safe disposal. The aim ofthe present study was to develop an economical method, so the dairy industry may adapt it to processwhey to make it suitable for safe disposal.Methods: The membrane chromatography was used to develop an economical method for the separationof whey proteins. Cellulose fiber treated with chlorosulfonic acid and commercially available adsorberSartobind? S was used to optimize the separation of whey protein isolate.Results: Dead end filtration study showed 1.02 mg / cm2 qm (maximum binding capacity) for cellulosefiber treated with chlorosulfonic acid and 1.3 mg / cm2 qm for Sartobind? S. Cellulose fiber showed2.7% decrease in adsorption at 30th cycle whereas 26% decrease in adsorption was obtained at 8th cyclefor Sartobind? S. The 19.0 fold higher surface area and 200 times higher feed volume was used forcross flow filtration study. It has showed 1.79 mg / cm2 qm for cellulose fiber and 3.2 mg / cm2 qm forSartobind? S. The average flux was 543 ± 36 l / m2 / h for cellulose fiber and it was 43 ± 0.0 l / m2 / hfor Sartobind? S. Electrophoresis of sample eluted at pH 7.0 showed three bands of major whey proteinsfor cellulose fiber whereas two bands for Sartobind? S. Cellulose fiber showed high potential for industrialapplications.Conclusion: In comparison to commercially available adsorber such as Sartobind? S, which has showedhigher whey protein binding capacity (qm). The cellulose fiber treated with CSA appears to be moresuitable for industrial applications. As it has shown higher flux (543 ± 36 l / m2 / h) and high flux regenerationcapacity with minimum washings that ensures its longer life.