Excision release of 5?hydroxycytosine oxidatively induced DNA DNA base lesions from the lung genome by cat dander extract challenge stimulates allergic airway inflammation

摘要

Summary Background Ragweed pollen extract ( RWPE ) induces TLR 4‐ NF κB‐ CXCL ‐dependent recruitment of ROS ‐generating neutrophils to the airway and OGG 1 DNA glycosylase‐dependent excision of oxidatively induced 8‐ OH ‐Gua DNA base lesions from the airway epithelial cell genome. Administration of free 8‐ OH ‐Gua base stimulates RWPE ‐induced allergic lung inflammation. These studies suggest that stimulation of innate receptors and their adaptor by allergenic extracts initiates excision of a set of DNA base lesions that facilitate innate/allergic lung inflammation. Objective To test the hypothesis that stimulation of a conserved innate receptor/adaptor pathway by allergenic extracts induces excision of a set of pro‐inflammatory oxidatively induced DNA base lesions from the lung genome that stimulate allergic airway inflammation. Methods Wild‐type ( WT ), Tlr4 KO , Tlr2 KO , Myd88 KO , and Trif KO mice were intranasally challenged once or repeatedly with cat dander extract ( CDE ), and innate or allergic inflammation and gene expression were quantified. We utilized GC ‐ MS / MS to quantify a set of oxidatively induced DNA base lesions after challenge of na?ve mice with CDE . Results A single CDE challenge stimulated innate neutrophil recruitment that was partially dependent on TLR 4 and TLR 2, and completely on Myd88, but not TRIF. A single CDE challenge stimulated MyD88‐dependent excision of DNA base lesions 5‐ OH ‐Cyt, FapyAde, and FapyGua from the lung genome. A single challenge of na?ve WT mice with 5‐ OH ‐Cyt stimulated neutrophilic lung inflammation. Multiple CDE instillations stimulated MyD88‐dependent allergic airway inflammation. Multiple administrations of 5‐ OH ‐Cyt with CDE stimulated allergic sensitization and allergic airway inflammation. Conclusions and Clinical Relevance We show for the first time that CDE challenge stimulates MyD88‐dependent excision of DNA base lesions. Our data suggest that the resultant‐free base(s) contribute to CDE ‐induced innate/allergic lung inflammation. We suggest that blocking the MyD88 pathway in the airways with specific inhibitors may be a novel targeted strategy of inhibiting amplification of innate and adaptive immune inflammation in allergic diseases by oxidatively induced DNA base lesions.