Proteomic response of the rat liver in differential swimming modes

摘要

Summary Moderate exercise helps improve competition results, providing a balanced muscle tone and biochemical activity, whereas excessive training disrupts the balance between training and recovery, causes harm to the organism, and leads to overtraining syndrome ( OTS ). To explore the mechanisms of different protein expressions during training and acquisition of immunity, we used proteomic analyses to investigate the differences of liver‐protein expressions between 2 swimming modes. Sprague‐Dawley rats were randomly divided into control ( CT ), fatigue training ( FT ), and exhaustive training ( ET ) groups, and liver tissues from each group were subjected to 2‐dimensional electrophoresis (2 DE ). A total of 4518 protein spots were detected in 9 replicates, and 45 protein spots exhibited a 2‐fold difference in expression ( P? ? .05), 31 of which was successfully identified by mass spectrometry. SERPINA 3K expression decreased markedly during 2 stages from CT ?→? FT and FT ?→? ET , while DDT , RHOT 1, and RBP 4 decreased significantly only from CT ?→? ET but not from the former 2 stages. By contrast, KRT 8, PCBD 1, KRT 18, PRDX 1, and ACY 1A showed significant 2‐fold increase in expression in either the CT ?→? FT or FT ?→? ET stages. Bioinformatic analyses showed that among the identified proteins, 30.2%, 54.18%, and 15.62% were involved in biological processes, molecular functions, and cell composition, respectively. Notably, PCBD 1, PRDX 1, and PPP 1 CB were involved in redox processes, while PPP 1 CB was only expressed in the FT group. RGN , PSMB 9, and AGT , commonly recognized as oxidative stress biomarkers, may involve in regulating homeostasis in the locomotor mode and may provide diagnostic criteria for the occurrence and prevention of exercise‐induced fatigue and OTS .