A comparative study of fine polished stainless steel, TiN and TiN/Ag surfaces: Adhesion and attachment strength of Listeria monocytogenes as well as anti-listerial effect

摘要

Magnetron sputtering was used to produce nanocomposite TiN and TiN/Ag coatings on stainless steel surfaces. The surface chemistry (EDX), physicochemical properties (contact angles), topography and roughness parameters (WLP and AFM) of the fine polished stainless steel (FPSS), TiN and TiN/8.6. at.% Ag surfaces were examined. Real-time initial adhesion of two Listeria monocytogenes strains (EGDe and 64) to the three surfaces was determined under flow conditions, and their attachment strength after adhesion was measured using atomic force microscopy (AFM). The anti-listerial properties of the surfaces were determined using LIVE/DEAD staining.Our results demonstrate that FPSS, TiN and TiN/8.6. at.% Ag possessed different surface properties, which may influence both attachment strength and anti-listerial properties. There were no significant (p> 0.05) differences in the initial adhesion of the two L. monocytogenes strains to the three different surfaces. Attachment studies showed that the two L. monocytogenes strains did not attach to FPSS under wetted conditions. However, both strains attached to TiN and TiN/8.6. at.% Ag surfaces, although with less strength to TiN/8.6. at.% Ag than to TiN surfaces. The TiN/8.6. at.% Ag surface showed marked anti-listerial properties as compared with FPSS and TiN. Initial adhesion, attachment strength and anti-listerial properties were found to be strain dependent.