首页> 外国专利> Primer set for loop-mediated isothermal amplification reaction for detecting Listeria monocytogenes, and method for detecting Listeria monocytogenes using the same

Primer set for loop-mediated isothermal amplification reaction for detecting Listeria monocytogenes, and method for detecting Listeria monocytogenes using the same

机译:用于检测单核细胞增生性李斯特氏菌的环介导的等温扩增反应的引物组以及使用其的检测单核细胞增生李斯特氏菌的方法

摘要

The present invention relates to a loop-mediated isothermal amplification reaction primer set for detecting listeria monocytogenes and a composition for detecting listeria monocytogenes using the same, and a kit and a detection method thereof. In the present invention, listeria monocytogenes can be specifically detected under higher sensitivity by performing the loop-mediated isothermal amplification reaction with use of the primer set of the present invention compared with a conventional method for detecting the same. In addition, contamination of listeria monocytogenes and the level thereof can be accurately checked fast by improving the reaction time due to performing the loop-mediated isothermal amplification reaction with use of the primer set of the present invention. Therefore, the primer set can be widely used for screening sample contaminated by listeria monocytogenes in an early screening system and for basic public health research.
机译:本发明涉及用于检测单核细胞增生李斯特氏菌的环介导的等温扩增反应引物组和使用其的用于检测单核细胞增生李斯特氏菌的组合物,试剂盒和检测方法。与常规的检测方法相比,在本发明中,通过使用本发明的引物组进行环介导的等温扩增反应,可以以更高的灵敏度特异性地检测单核细胞增生李斯特菌。另外,由于通过使用本发明的引物组进行环介导的等温扩增反应,通过改善反应时间,可以快速准确地检查单核细胞增生性李斯特菌的污染及其水平。因此,该引物组可广泛用于在早期筛查系统中筛查被单核细胞增生李斯特菌污染的样品,并用于基础公共卫生研究。

著录项

  • 公开/公告号KR101496825B1

    专利类型

  • 公开/公告日2015-02-27

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20140025600

  • 发明设计人 조성범;조애리;동희진;

    申请日2014-03-04

  • 分类号C12N15/11;C12Q1/68;

  • 国家 KR

  • 入库时间 2022-08-21 14:58:34

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