Preparation of graphene oxide doped eggshell membrane bioplatform modified Prussian blue nanoparticles as a sensitive hydrogen peroxide sensor

摘要

This study describes the preparation and characterization of graphene oxide doped eggshell membrane (GO-ESM) as a novel electrochemical bioplatform for electroanalytical purposes. The GO-ESM bioplatform was prepared by incorporation of GO nano-sheets into the ESM via a facile sonication procedure. Field emission scanning electron microscopy and X-ray diffraction powder techniques were used to characterize the developed bioplatform. The electrochemistry of GO-ESM was investigated by decorating it on the surface of carbon ceramic electrode (CCE) by an O-ring. The GO-ESM platform was modified with Prussian blue (PB) via a facile dip-coating method. Then the resulted modified electrode (PB|GO-ESM|CCE) was used as a novel hydrogen peroxide electrochemical sensor. The fabricated electrode responds efficiently to H_2O_2 over the concentration range 125nM-195 μM with a detection limit of 31 nM (S/N = 3) and sensitivity 8.8 μA μM~(-1) cm~(-2). The PB|GO-ESM|CCE has been successfully applied to determination of H_2O_2 content in spiked milk samples. Due to good stability, environmental friendly, cheapness, nontoxic, well behaved electrochemical properties, and biocompatibility, the fabricated bioplatform has the promising future for practical applications.