Occurrence and identification of Impatiens necrotic spot tospovirus in the Czech Republic.

摘要

Impatiens necrotic spot virus (INSV) was detected for the first time in April 1999 in a glasshouse in Central Bohemia in imported Columnea sp. and Curcuma sp. plants. Three months later INSV was found in another garden centre in Central Bohemia in Chrysanthemum morifolium, Begonia tuberhybrida, Aeschynanthus sp., Hippeastrum hortorum and Verbena hybr. Temari. In January 2000 INSV was detected in a glasshouse in South Bohemia and in March 2000 in a glasshouse in South Moravia. The naturally infectedornamental plant species were: Anthurium andreanum, Asplenium nidus, Begonia peltata, Cyrtomium falcata, Kalanchoe blossfeldiana, Osteospermum sp., Philodendron sp., Saxifraga stolonifera and Spathiphyllum floribundum. In these glasshouses INSV infection was found also in Stellaria media which is an important and most common weed plant species. Determination of thrips in sites of INSV infection revealed that Frankliniella occidentalis was present. INSV was identified by enzyme-linked immunosorbent assay (ELISA) using polyclonal antibodies to INSV from Loewe Biochemica, Germany and from Bioreba, Switzerland. Sap from the infected plant species was used for mechanical inoculations of indicator plants. Nicotiana benthamiana developed characteristic symptoms of INSV infection; Datura stramonium and Solanum stramonifolium reacted with local lesions, systemic mosaic and leaf deformation. The sap extracts of these infected indicator plants gave strong positive reactions in ELISA. Leaf homogenates of indicator plants were analysed by transmission electron microscopy. Spherical enveloped virus particles typical of a Tospovirus were detected.