Structure of the O-specific polysaccharide from a halophilic bacterium Halomonas ventosae RU5S2EL

摘要

Halomonas ventosae RU5S2EL, a halophilic Gram-negative bacterium isolated from salt sediments of lake Elton (Russia), was cultivated and the lipopolysaccharide was extracted by the Westphal procedure. The O-specific polysaccharide (OPS) was obtained by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with H-1 and C-13 NMR spectroscopy, including H-1, H-1 COSY, TOCSY, ROESY, H-1, C-13 HSQC, and HMBC experiments as well as Smith degradation. The OPS was found to consist of branched pentasaccharide repeating units of the following structures: