Lipopolysaccharide of Pantoea agglomerans 7460: O-specific polysaccharide and lipid A structures and biological activity

摘要

Lipopolysaccharide (LPS) was isolated from Pantoea agglomerans 7460 cells by phenol-water extraction. Mild acid degradation allowed to separate OPS and lipid A. Lipid A was analyzed by negative-ion mode ESI MS and found to consist mainly of hexaacylated derivative containing biphosphorylated G1cN disaccharide, four 14:0 (3 -OH), 18:0 and 12:0 fatty acids. The structure of the O-specific polysaccharide was established by chemical, NMR and computational methods: -> 2)alpha-D-Rhap-(1 -> 2)-alpha-D-Rhap(1 -> 4)-beta-D-Galp-(1 ->alpha-D-Galp-(1 -> 3)-(1) similar to 70%