阴外动脉灌注脂多糖对泌乳奶牛动脉血液中脂肪酸组成、摄取及血液脂质指标的影响

摘要

This paper was designed to evaluate plasma fatty acids profile, uptake and lipid indexes in response to LPS challenge following intra-external pudic arterial in dairy cows. Six multiparous, lactating Holstein cows received a single, small dose of LPS (Escherichia coli O111:B4) in 10 mL of sterile saline (0.9% NaCI) solution (0.01 ug ? Kg'1 BW) or sterile saline solution alone (control treatment) in one of a two-week randomized crossover study. The results showed that plasma fatty acids profile was not significantly affected (P>0.05), but tented to increase the content of T-FA, SFA, SLFA and CLFA. Compared to the control, LPS challenge increased mammary gland uptake for the length of carbon chain more than sixteen in various degrees (P>0.05) excepted for C20 (P<0.01). The challenge also increased the plasma content of CHOL (P<0.05), HDLD (P>0.05) and LDLD (P>0.05). It could be concluded that LPS mobilized lipid metabolism for the inflammation.%研究阴外动脉灌注脂多糖(LPS)对泌乳奶牛动脉血液中脂肪酸组成、摄取及血液脂质指标的影响.选用6头经产的荷斯坦奶牛(BW=(576.33±36.67)kg,DIM=(185.67±30.11)d),随机分成2组.采用交叉试验设计,每期试验7d,间隔期14d；试验组阴外动脉灌注LPS(Escherichia coli 0111:B4,0.01 μg·kg-1体重),对照组阴外动脉灌注生理盐水.结果表明,阴外动脉灌注LPS未显著影响动脉血浆中脂肪酸的组成(P＞0.05),但有增加血浆中T-FA量、SFA含量、SLFA含量和CLFA含量的趋势.与对照组相比,LPS组不同程度的提高了乳腺对碳链长度大于16的脂肪酸摄取率,除对C20的摄取率达到极显著水平(P＜0.01)外,对其余脂肪酸的摄取率影响差异不显著(P＞0.05).与对照组相比,试验组动脉血浆中CHOL含量显著提高(P＜0.05),HDLD含量和LDLD含量有提高的趋势(P＞0.05).结果显示,LPS能动员机体的脂类代谢,以抵抗LPS对动物机体诱发的炎症反应.