Moving beyond preparative reverse phase HPLC for peptide purification

摘要

Today's peptide chemist has at his or her disposal a wide variety of chemistries to help make peptide synthesis more effective. There is a seemingly endless choice of coupling agents to help difficult couplings, or orthogonally reactive protecting groups to enable peptide side chain modification. But the methodology for purification has not been developed so readily - by far, the greatest reliance remains on preparative reverse phase HPLC (I). This is not because other techniques do not exist, but they have not been so readily adopted. This article examines the options beyond HPLC when it comes to peptide purification.