Glutamate as an Efficient Amine Donor for the Synthesis of Chiral beta- and gamma-Amino Acids Using Transaminase

摘要

A recyclable glutamate amine donor system employing transaminase (TA), glutamate dehydrogenase (GluDH) and mutant formate dehydrogenase (FDHm) was developed, wherein amine donor Glu was regenerated using GluDH and thereby circumvented the inhibition of TA by alpha-ketoglutarate. Various enantiopure beta-, gamma-amino acids, and amines were successfully synthesized with high conversions and excellent enantiomeric excess using this system.