Co‐immobilized Whole Cells with ω‐Transaminase and Ketoreductase Activities for Continuous‐Flow Cascade Reactions

Nagy‐Gy?r László; Abaházi Emese; Bódai Viktória; Sátorhelyi Péter; Erdélyi Balázs; Balogh‐Weiser Diána; Paizs Csaba; Hornyánszky Gábor; Poppe László

摘要

Abstract > An improved sol–gel process involving the use of hollow silica microspheres as a supporting additive was applied for the co‐immobilization of whole cells of Escherichia coli with Chromobacterium violaceum ω‐transaminase activity and Lodderomyces elongisporus with ketoreductase activity. The co‐immobilized cells with two different biocatalytic activities could perform a cascade of reactions to convert racemic 4‐phenylbutan‐2‐amine or heptan‐2‐amine into a nearly equimolar mixture of the corresponding enantiomerically pure R amine and S alcohol even in continuous‐flow mode. The novel co‐immobilized whole‐cell system proved to be an easy‐to‐store and durable biocatalyst. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>摘要</标题> >施加涉及使用中空二氧化硅微球作为支撑添加剂的改进的溶胶 - 凝胶工艺用于将大肠杆菌的全细胞共固定，具有浓度的紫杉杆菌，ω-转氨酶活性和 Lodderomyces Elongisporus患者，具有酮还原酶活性。具有两种不同的生物催化活性的共固化细胞可以进行级联反应，以将外消旋4-苯基丁丹-2-胺或庚烷-2-胺转化为相应对映体纯度的几乎等摩尔混合物R </ i> 即使在连续流动模式下也是胺和 s </ i>醇。新型共同固定的全细胞系统被证明是一种易于储存和耐用的生物催化剂。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-16039/'>《Chembiochem: A European journal of chemical biology》</a> <b style="margin: 0 2px;">|</b><span>2018年第17期</span><b style="margin: 0 2px;">|</b><span>共4页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nagy‐Gy?r László&option=202" target="_blank" rel="nofollow">Nagy‐Gy?r László;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Abaházi Emese&option=202" target="_blank" rel="nofollow">Abaházi Emese;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bódai Viktória&option=202" target="_blank" rel="nofollow">Bódai Viktória;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sátorhelyi Péter&option=202" target="_blank" rel="nofollow">Sátorhelyi Péter;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Erdélyi Balázs&option=202" target="_blank" rel="nofollow">Erdélyi Balázs;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Balogh‐Weiser Diána&option=202" target="_blank" rel="nofollow">Balogh‐Weiser Diána;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Paizs Csaba&option=202" target="_blank" rel="nofollow">Paizs Csaba;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hornyánszky Gábor&option=202" target="_blank" rel="nofollow">Hornyánszky Gábor;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Poppe László&option=202" target="_blank" rel="nofollow">Poppe László;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsM?egyetem rkp. 3 1111 Budapest Hungary;</p> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsM?egyetem rkp. 3 1111 Budapest Hungary;</p> <p>Fermentia Microbiological Ltd.Berlini út 47–49 1045 Budapest Hungary;</p> <p>Fermentia Microbiological Ltd.Berlini út 47–49 1045 Budapest Hungary;</p> <p>Fermentia Microbiological Ltd.Berlini út 47–49 1045 Budapest Hungary;</p> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsM?egyetem rkp. 3 1111 Budapest Hungary;</p> <p>Biocatalysis and Biotransformation Research CentreBabe?-Bolyai University of Cluj-NapocaArany János Str. 11 400028 Cluj-Napoca Romania;</p> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsM?egyetem rkp. 3 1111 Budapest Hungary;</p> <p>Department of Organic Chemistry and TechnologyBudapest University of Technology and EconomicsM?egyetem rkp. 3 1111 Budapest Hungary;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/179.html" title="分子生物学">分子生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=continuous flow&option=203" rel="nofollow">continuous flow;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=kinetic resolution&option=203" rel="nofollow">kinetic resolution;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=oxidoreductases&option=203" rel="nofollow">oxidoreductases;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=transferases&option=203" rel="nofollow">transferases;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=whole-cell immobilization&option=203" rel="nofollow">whole-cell immobilization;</a> </p> <div class="translation"> 机译：连续流动;动力学分辨率;氧化还原酶;转移酶;全细胞固定化; </div> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div class="reference_details" id="referenceList"> <ul id="referenceul"></ul> <ul id="citationul"></ul> <ul id="commonreferenceul"></ul> <ul id="commoncitationul"></ul> <ul id="tworeferenceul"></ul> <ul id="twocitationul"></ul> </div> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704022055746.html">Front Cover: Co‐immobilized Whole Cells with ω‐Transaminase and Ketoreductase Activities for Continuous‐Flow Cascade Reactions (ChemBioChem 17/2018)</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nagy‐Gy?r László&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Nagy‐Gy?r László,</a> <a 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