Expression of apoptosis markers on umbilical cord blood (UCB) CD34+cells after 10% and 5% dimethylsulfoxide (DMSO) cryopreservation

摘要

UCB is being used as a source of hematopoietic stem cells for the bone marrow reconstitution and commonly is cryopreserved with 10% DMSO. The aim of our study was to evaluate the viability and apoptosis by flow cytometry on UCB CD341 cells comparing 10% and 5% DMSO cryopreservation. After red blood cell depletion and positive selection of CD341 cells, UCB samples (n 5 8) were divided in equal volumes and cryopreserved either with 10% or 5% DMSO.