D-galactose induced inflammation lipid peroxidation and platelet activation in rats

摘要

Background: To investigate events possibly related to the development of d-galactose induced senescence, we examined whether 8-iso PGF2α formation, a marker of in vivo lipid peroxidation is altered and whether its biosynthesis is associated with 11-dehydro-TXB2 excretion rate, as a marker of in vivo platelet activation. In this setting, we also investigated the relationship between proinflammatory mediators (IL-6 and TNF-α from one, and lipid peroxidation and platelet activation, from another aspect. Methods and results: Forty animals were divided, depending on treatment with d-galactose into: placebo and d-galactose treated rats. 8-iso-PGF2α, IL-6 and TNF-α were measured in plasma, while 11-dehydro-TXB2 was determined in the urine after a six week treatment with d-galactose. Compared to placebo, d-galactose treated animals showed significantly higher levels of all measured parameters. Conclusions: d-galactose induced changes in the rate of F2-isoprostane formation are associated with the changes in the excretion rate of 11-dehydro-TXB2.