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Genomic organization, expression and evolution of porcine CRSP1,2, and 3

机译:猪CRSP1,2和3的基因组组织,表达和进化

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Recently we identified and characterized porcine calcitonin receptor-stimulating peptide (CRSP) 1, l"RSP2 and CRSP3 as members of the calcitonin/calcitonin gene-related peptide (CT/CGRP) family. In the present -study, the genomic sequences and organization of CRSP1,2, iMid 3 were determined, and the expression of the genes in the porcine brain was investigated using in situ hybridization. Analysis of 5'-upstream regions of the three CRSPs demonstrated that CRSP1 and CRSP2 have almost identical sequences (>98% similarity) and high sequence similarities including functional transcription binding sites with the corresponding region of human CALCA (CT/aCGRP), whereas CRSP3 retains less similarity with the above genes. RH mapping of CRSPs demonstrated that they resided in a region of swine chromosome 2 (SSC2). The arrangement of the genes in the region was found to be conserved in corresponding human and mouse regions. In situ hybridization demonstrated sense transcripts of the three genes in cerebrum,hippocampus, hypothalamus, pons/midbrain, and thalamus of 3-month-old pigs, and CRSP2 sense transcripts additionally in tractus opticus. The sense transcripts of aCGRP and CALCB (/3CGRP) were detected in cerebrum, hippocampus, and pons/midbrain of newborn mice, and to a lesser extent in pons/midbrain of 8-week-old mice. These results taken together with the chromosomal conservation and phylogenetic clustering of CT/CGRP family indicate that CRSP1, 2, and 3 may be functionally different from alpha CGRP and beta CGRP, though they are indicated to have acommon progenitor gene.
机译:最近,我们鉴定和表征了猪降钙素受体刺激肽(CRSP)1、1“ RSP2和CRSP3是降钙素/降钙素基因相关肽(CT / CGRP)家族的成员。在本研究中,基因组序列和组织确定CRSP1,2的iMid 3,并用原位杂交技术研究猪脑中基因的表达,对三个CRSP 5'上游区域的分析表明,CRSP1和CRSP2具有几乎相同的序列(> 98) %相似性)和高序列相似性,包括与人CALCA相应区域(CT / aCGRP)的功能性转录结合位点,而CRSP3与上述基因的相似性较低。CRSPs的RH定位表明它们位于猪2号染色体区域(SSC2)。发现该区域中基因的排列在相应的人和小鼠区域中是保守的,原位杂交证实了这三个基因的有义转录本在3个月大的猪的大脑,海马,下丘脑,脑桥/中脑和丘脑中,以及视神经束中CRSP2有义转录本。在新生小鼠的大脑,海马和脑桥/中脑中检测到aCGRP和CALCB(/ 3CGRP)的有义转录本,而在8周龄小鼠的脑桥/中脑中检测到的转录本较小。这些结果以及CT / CGRP家族的染色体保守性和系统发生聚类结果表明,CRSP1、2和3在功能上可能不同于alpha CGRP和beta CGRP,尽管它们表明它们具有共同的祖基因。

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