Validation and application of a novel UHPLC–MS/MS method for the measurement of furanodienone in rat plasma

摘要

Abstract A sensitive ultra‐high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method was established to analyze furanodienone in rat plasma. In the process of chromatographic separation, selected reaction monitoring transitions for furanodienone and patchouli alcohol (internal standard, IS) were m/z 231.1?→?83.2 and m/z 205.1?→?95.1, respectively. Great linearity of furanodienone in plasma samples was found in the corresponding concentration range ( r ??0.995). Intra‐ and inter‐day precisions (RSD, %) were 11.3% in plasma, and the accuracy (RE, %) was within ±10.7%. This method was used to the furanodienone study on rat pharmacokinetics after a single oral dose of 10?mg/kg of furanodiene. The results indicated that the maximum observed plasma concentration was 52.4?±?19.1?ng/ml at 1.2?±?0.7?h with an elimination half‐life of 2.2?±?0.7?h. The obtained data indicated that furanodienone could be moderately distributed and eliminated.