首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >A novel freeze-dried storage and preparation method for the determination of mycophenolic acid in plasma by high-performance liquid chromatography
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A novel freeze-dried storage and preparation method for the determination of mycophenolic acid in plasma by high-performance liquid chromatography

机译:高效液相色谱法测定等离子体中霉苯烯酸测定的新型冷冻干燥储存及制备方法

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摘要

Plasma samples were conventionally stored at freezing conditions until the time of detection. Such a technique, when carried out over an extended period, is energy consuming; in addition, preparation and transportation of stored samples is inconvenient. In this study, a freeze-dried storage and preparation method was proposed to determine the presence of mycophenolic acid (MPA) in plasma. Fresh plasma samples were freeze-dried using a device, and then stored at ambient temperature. After the stored samples were soaked with methanol spiked with the internal standard, high-performance liquid chromatography was conducted to detect MPA. The proposed method was demonstrated to be precise and accurate over the linear range of 0.5-50 mu g mL(-1), with both intra- and inter-day precision being <7% and biases <10%. The freeze-dried samples were stable at ambient temperature for at least 40 days. This method was also successfully applied to the pharmacokinetic study of MPA in healthy volunteers. Pharmacokinetic parameters, such as maximum plasma concentration, time point of maximum plasma concentration and elimination half-life, among others, were consistent with the results in the published study. This proposed technique was proved to be simple, reproducible and energy saving. This approach could also simplify the storage and analysis of samples in clinical and scientific drug research.
机译:常规储存等离子体样品在冷冻条件下,直至检测时间。这种技术在延长时期进行时,是能耗;此外,储存样本的制备和运输是不方便的。在该研究中,提出了一种冷冻干燥的储存和制备方法,以确定血浆中霉菌酸(MPa)的存在。使用装置冷冻干燥新鲜的等离子体样品,然后在环境温度下储存。将储存的样品用甲醇浸泡在掺入内标甲醇后,进行高效液相色谱法检测MPa。所提出的方法被证明在0.5-50μmgml(-1)的线性范围内精确,精确,具有<7%和偏倚<10%的内部精度。冷冻干燥的样品在环境温度下稳定至少40天。该方法还成功地应用于健康志愿者MPa的药代动力学研究。药代动力学参数,如最大血浆浓度,最大血浆浓度的时间点,除了另外的半衰期之外,与发表研究的结果一致。证明这一提出的技术简单,可重复和节能。这种方法还可以简化临床和科学研究中样品的储存和分析。

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